A cart was brought out for Williams in the third quarter, but he elected to limp off the field with some assistance to the locker room. He was ruled out in the fourth quarter and if tests confirm Williams did suffer a high ankle sprain, the standout tackle would likely miss some time.
Williams, regarded as arguably the best left tackle in the NFL, was injured when he was blocking Broncos defensive end Bradley Chubb, and Denver defensive lineman D.J. Jones was blocked to the ground and fell across Williams' right leg. Quarterback Jimmy Garoppolo stepped out of the end zone for a safety on the play.
Williams was replaced by Jaylon Moore. Chubb registered his first and only sack of the night late in the game going against Moore with Williams in the locker room.
A nine-time Pro Bowler, the 34-year-old Williams is in his third season with the 49ers and is coming off an All-Pro campaign. Williams was voted No. 14 in the NFL Network's annual Top 100 Players, the highest ranking ever achieved by an offensive lineman.